I will repeat what others have said. You need at least 50 hours on the tubes and lots of listening times. I like to listen during breakin at least periodically for the changes. Tubes are a bit like gardening. It is a slow process to see or hear change.
I recommend new tubes. They are making news tubes specifically for audio and honestly, I think they are better than playing the made 50 years ago NOS game. IMO, new audio tubes made with modern design tools and equipment are more accurate than a tube made 50 years ago for a radar or basic radio. Tubes already add in tube flavor and many NOS go too far into tube land. Now if you need or want that go for it, but I prefer the accuracy of new tubes. For small signal tubes, I have found Rays Reserve and Gold Lion Gold Pin to be the best in the 12AU7 tubes. Great clarity and still the holographic sound of tubes. I find 40 to 50 hours is needed to for break in for my ears on my equipment - might get better beyond that, but if so, it would be small and I am on to different upgrades. Finally, my tube preamp did not like being paired with the Gaia isolation feet - the sound was flat and without sparkle and I lost soundstage.

