Do isolation devices really help?

Yes they help. Warren is correct in that they do not have much effect and can actually work against air born motion, and in some cases coupling is the way to go here. As has already been mentioned different devices will have different effects on different equipment. Some subtle, some large, some will even make things sound worse. In general you have to experiment to figure out what type of devices work best for what pieces in your system.
Another generalization is that coupling speakers to the floor cleans up the soundstage, tightens the base. This is in line with what Warren has said. The speaker is creating air born motion and you would like it to remain rigid to create that accurately. Now, that said, there are those that use vibrapods or roller bearings under their speakers and swear by them. These devices have not worked for me for speakers, but I obviously haven't heard these other systems.
Other devices produce internal vibration. A CD transport is an obvious one and these frequently benefit by absorbing that mechanical energy with vibrapods. (again a generalization)
My amplifiers have huge transformers in them that have a 60Hz mechanical oscilation (all transformers do to some extent--but the really large ones are worse). I use a roller bearing system for these. It couples the transformer to the floor, but allows the 60Hz energy to be disappated laterally. This has been an extremely effective isolation device for me.
My examples show you three types of devices that work differently: absorbing energy such as vibrapods, coupling, such as spikes, and coupling with disappation, roller balls. Shelving can also make a difference, and I've been using a combination of granite and corian.
I do have a friend that has the ultimate isolation device. All the equipment is in a separate room from the speakers. Of course it's all isolated independently in the separate room.

Try Aurios under your CD player (I recommend the 1.2s). Then YOU answer the question.

One isolation product made a complete difference under my tube pre. A Symposium Ultra shelf. Expensive , yes, but an amazing improvement. Literally, I woudl have to change the component to hearas muchadifffence. It took my BC3 Galatea to another level. If this makes sense, it was more quiet, more dynamic and more extended. Amazing prduct that I highly recomend under anything tube. I am going to try it under my Meridian player next. I am currently using Sympoium Rollerblocks under my Meridian and the change was subtle but better han any cone I have used. The highs were more extended, dynamics improved and low level bass improved a smidge. I highly reccomend you try out the stuff from Symposium!

Where does all the air born resonances go to after they get into your electronics? How do they escape? It would seem that these isolation devices, in themselves, help keep those resonances right where you don't want them. You want all resonances to escape and go to the floor. Everything should, rack as well, be coupled with cones. I use Audio points, and am very happy. Sistrum Rack System does this (resonance transference) better than any I know of, as do their speaker support systems.

Yes! I have #2 Vibrapods under Amp and CDP and the sound is just clearer, more focused. Short story, I originally put mouse pads under the Vibrapods and while it improved the sound (versus w/out them) it was still lacking something. Clarity and detail to be precise. After reading A'gon I came to the conclusion that the mouse pads under the Pods was overkill. Went out and bought bath tile, put them under the Pods and "low and behold" there was my clarity and detail that was missing.

So, such isolation devices do matter.

Matty