100 hz should be the minimum. Anything above that becomes directional to the ear.
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To determine the best crossover point by ear start by following the instruction given by the manufacture, then place the sub at the the listening position and follow the procedure I have described here:
Looking for the BASS
Some additional tips for setting up the sub you can find here:
SubWoofer Help ASAP...
Of course there are quite a few other threads here about setting up a subwoofer. So use the search function for more tips.
Good luck and keep us posted!