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I can't see how a cables' position under or on the floor could possible make a difference. In my mind, having it under the floor(which is what I am doing), is preferable, to lying on the floor. As Stringreen said, is it near any AC wires?
Wow, I wish I only had 2.5 meters between the speakers and amp.
My 22 foot long speaker cables are under the floor and my system sounds just wonderful. I have never had issues with any kind of noise. Why go through the trouble of running a conduit? Just lay em between the rafters and be done with it.. Just remember to leave some slack in the cables at both ends where it goes through the floor.
I had 40 feet of interconnect under the floor of my old house. Since it was a 12 foot deep basement under the floor, I was able to suspend the cables from the basement ceiling with silicone coated rubber bands suspended from hooks in the beams, which did a pretty good job of isolating the cables from vibrations (more important with interconnects than speaker cable, but something you might want to keep in mind). Did not need a conduit for them due to the depth of the basement--don't know your situation.
In my last installation I ran 35' of interconnect in the crawl space with no issues but one thing to bear in mind is making the entry/exit to the floor look nice while still keeping creepy crawlies out -- I found these products used in clean rooms to be a good thing to use
(search for brush grommets)