Does hotglue affect the ...

dielectric properties of a speaker cable, if used to physically confine cables within the XLR connectors on both ends? Note that the point of contact is with the bare uninsulated parts of the cable. To be specific, the juncture where the wire is soldered onto the XLR pin.

I was terminating a pair of speaker wires into their Neutrik XLR connectors and used hotglue between the wires as insurance against a short-circuit and a destroyed amplifier if, for some unforseen reason, one of the wires should ever decide to come off and stray across to the opposite polarity. That way, the hotglue would serve to keep the wires physically apart.

Now, I know for a fact that hotglue becomes conductive above 600VDC. (A hair-thin strand that happened to be between the 2 output terminals of my 600V DC PhotoFlash Capacitor Bank when it was beginning to be charged up, taught me that interesting fact, but I digress (again)...)

Now, I am wondering if having the hot glue between bare conductors in a XLR connector will give rise to any "ghost" sonic signatures (e.g. acts as a capacitor because of its higher dielectric property? Conducts a minute current at a specific frequency?)

Your expertise is highly appreciated.

Very, very happy with his upgraded speaker cables on his VideoLogic Sirocco Pro,
You might try electrical grade silicone, and ONLY electrical grade. This will also help dampen vibration in connector.
You are way too paranoid. A little hot glue will not affect anything. It will increase the capacitance and the dielectric absorption microscopically. Neither of these is a first-order effect in a speaker cable. Better to protect your gear.